Sexually Transmitted Diseases

Abstract:

A comprehensive collection of cutting-edge molecular protocols for the laboratory diagnosis and study of

sexually transmitted diseases, including HIV. The methods—each crafted by a leading expert for

comprehensiveness and genuine laboratory utility—cover the major sexually transmitted pathogens, including

Neisseria gonorrhoeae, Chlamydia trachomatis, Haemophilius ducreyl, Treponema pallidum, and herpes

simplex. Additional molecular techniques are included for detecting the genital mycoplasmas, hepatitis B virus,

HIV-1, human papillomavirus. Sexually Transmitted Disease Protocols provides a comprehensive collection of

foolproof molecular methods for STD diagnosis, research, and patient management.

Contents:

1. The Impact of Molecular Technology on STD Control: A Historical Perspective
P. Sparling
pp. 3-11

2. Neisseria gonorrhoeae: Detection and Typing by Probe Hybridization, LCR, and PCR
Charlotte Gaydos; Thomas Quinn
pp. 15-32

3. Molecular Diagnosis of Chlamydia trachomatis Infections by Probe Hybridization, PCR, LCR, TMA, and Q-â

Replicase
Max Chernesky; James Mahony
pp. 33-46

4. Haemophilus ducreyi Detection by PCR
Patricia Totten; Jane Kuypers; Stephen Morse
pp. 47-66

5. Detection of Treponema pallidurn, Haemophilus ducreyi, and Herpes Simplex Virus by Multiplex PCR
Karina Orle; Judith Weiss
pp. 67-80

6. Detection of Genital Mycoplasmas by PCR
Claire Gilroy; David Taylor-Robinson
pp. 81-102

7. Hepatitis B Virus: Detection and Quantitation by Membrane and Liquid Hybridization, Branched DNA Signal

Amplification, Hybrid Capture, and PCR Methods
Mel Krajden
pp. 103-128

8. Molecular Diagnosis of HIV-1 by PCR
Susan Fiscus
pp. 129-139

9. Genotyping of Neisseria gonorrhoeae by Pulse Field Gel Electrophoresis and PCR
Ian Maclean
pp. 143-150

10. Genotyping Chlamydia trachomatis by PCR
Deborah Dean

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