Molecular Diagnosis of Infectious Diseases

A comprehensive and up-to-date set of basic and advanced methods that illuminates the principles of molecular diagnostics and allows researchers to establish assay systems tailored to their specific needs. This unique collection has been thoroughly updated and expanded and covers the latest strategies for identifying novel diagnostic markers, epitope mapping, and the production and purification of recombinant antigens and their use as diagnostic reagents in immunological assays. This new edition places significant emphasis on bioinformatics and proteomics and the characterization and modification of proteins. It also includes promising techniques based on recent developments in antibody engineering and on the use of biosensors. Rather than focusing on particular infectious agents, this book focuses on the techniques that can be used and tailored to accomplish any kind of molecular diagnostic application in the clinical laboratory, thus making it an essential book for all laboratory disagnosticians.

Contents:
1. Identification of Novel Diagnostic Markers by Differential Display
Peng Liang; Fellan Wang; Welmin Zhu; Robert O'Connell; Lidia Averboukh
pp. 3-13

2. lmmunoprecipitation
Kari Johansen; Lennart Svensson
pp. 15-28

3. RTPCR Methods and Applications
Paul Siebert
pp. 29-54

4. Quantitative RT-PCR
Paul Siebert
pp. 55-80

5. Cloning cDNA Ends Using RACE
Yue Zhang; Michael Frohman
pp. 81-106

6. A Method for the Rapid Identification of Epitopes and Other Functional Peptide Domains
Robert Mierendorf; Beth Hammer; Robert Novy
pp. 107-124

7. Mapping of Linear B-Cell Epitopes on Viral Polypeptides by Multiple Peptide Synthesis and Fine Tuning Sensitivity and Specificity of the Identified Peptide Antigens for Application in Virus Diagnosis
Ulla Wienhues; Wolfgang Kraas; Christoph Seidel; Hans Ihlenfeldt; Günther Jung
pp. 125-142

8. Amplification of Genomic DNA by PCR
Bruno Frey
pp. 143-156

9. PCR-Based Cloning and Subsequent _Expression of Antigenic Proteins in Escherichia coli
Udo Reischl
pp. 157-168

10. Application of TEV Protease in Protein Production
Deborah Polayes; T. Parks; Stephen Johnston; William Dougherty
pp. 169-184


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