Absctact:
Expert scientific and clinical investigators present proven human cell culture techniques applicable to tissue samples taken from a wide variety of organs, particularly those prone to pathological change. They describe in clear, step-by-step instructions the special requirements for successfully culturing such human cells as T-cells, trophoblast cells, renal cells, natural killer cells, endothelial cells, neurons, epithelial cells, pituitary cells, and more. The protocols eliminate much of the chore of adapting techniques initially developed for animal cell culture systems or the time spent in hunting down potentially useful techniques buried in the details of research papers, or even in books largely devoted to animal cell culture. They allow researchers to use successfully high quality in vitro cultures as models to explore the disease process.
Table of contents:
Establishment and Maintenance of Normal Human Keratinocyte Cultures
Cultivation of Normal Human Epidermal Melanocytes
Cultivation of Keratinocytes from the Outer Root Sheath of Human Hair Follicles
Isolation and Short-Term Culture of Human Epidermal Langerhans' Cells
Growth and Differentiation of Human Adipose Stromal Cells in Culture
Human Fetal Brain Cell Culture
Culture of Human Normal Brain and Malignant Brain Tumors for Cellular, Molecular, and Pharmacological Studies
Cell Culture of Human Brain Tumors on Extracellular Matrices: Methodology and Biological Applications
Isolation and Culture of Human Umbilical Vein Endothelial Cells
Human Thymic Epithelial Cell Cultures
Generation and Cloning of Antigen-Specific Human T-Cells
Protection of Purified Human Hematopoietic Progenitor Cells by Interleukin- 1b or Tumor Necrosis Factor-a
Human Mononuclear Phagocytes in Tissue Culture
Purification of Peripheral Blood Natural Killer Cells
Cystic Fibrosis Airway Epithelial Cell Culture
Gene Transfer into Cystic Fibrosis Airway Epithelial Cells
Genetic Analysis of Cystic Fibrosis Airway Epithelial Cells
Human Tracheal Gland Cells in Primary Culture
Human Chondrocyte Cultures as Models of Cartilage-Specific Gene Regulation
Isolation and Culture of Bone-Forming Cells (Osteoblasts) from Human Bone
The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures
In Vitro Cellular Systems for Studying Osteoclast Functionand Differentiation: Primary OC and the FLG 29.1 Model
Isolation, Purification, and Growth of Human Skeletal Muscle Cells
Cultures of Proliferating Vascular Smooth Muscle Cells from Adult Human Aorta
Human Myometrial Smooth Muscle Cells and Cervical Fibroblasts in Culture: A Comparative Study
Primary Culture of Human Antral Endocrine Cells
Primary Cell Culture of Human Enteric Neurons: Submucosal Plexus
Isolation and Culture of Human Hepatocytes
Culture of Human Pancreatic Islet Cells
Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
Glomerular Epithelial and Mesangial Cell Culture and Characterization
Enzymatic Isolation and Serum-Free Culture of Human Renal Cells: Retaining Properties of Proximal Tubule Cells
Culture of Human Renal Medullary Interstitial Cells
Culture Technique of Human Pituitary Adenoma Cells
Preparation of Human Trophoblast Cells for Culture
Superfused Microcarrier Cultures of BeWo Choriocarcinoma Cells: A Dynamic Model for Studies of Human Trophoblast Function
Corneal Organ Culture
Keratocyte Cell Culture
Conjunctiva: Organ and Cell Culture
Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium
Demonstration of Mycoplasma Contamination in Cell Cultures by a Mycoplasma Group-Specific Polymerase Chain Reaction
http://rapidshare.de/files/34005889/002-Human_Cell_Culture_Protocols.pdf
